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标题：CHARACTERIZATION OF CAROTENOID PIGMENTS FROM BACTERIAL SYMBIONTS OF SEAGRASS Thalassia hemprichii
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作者：Sri Achadi Nugraheni ; Miftahuddin M Khoeri ; Lia Kusmita 等
期刊名称：JOURNAL OF COASTAL DEVELOPMENT
印刷版ISSN：1410-5217
出版年度：2010
卷号：14
期号：1
页码：51-60
语种：English
出版社：JOURNAL OF COASTAL DEVELOPMENT
摘要：Carotenoids are pigments that can be used in various applications including cosmetics and precursor of vitamins A. Carotenoids are mostly found in higher plant leaves, fruit, and bacteria. Marine bacteria associated with seagrass Thalassia hemprichii collected from Menjangan Kecil Waters, Karimunjawa Islands were screened to produce the pigment and has allowed the use of these microrganism as an environmental friendly alternative source of new natural pigment. The isolation of bacterial symbionts on Zobell 2216E medium from seagrass Thalassia hemprichii resulted in 20 isolates of which 8 bacterial symbionts have produced pigments but only one bacterium positively synthesize carotenoids. Initial analysis with atomic absorption spectrophotometric method revealed that the wave lengt of bacterial pigment were in the range of 300-600 nm, which are categorized that within the group of carotenoid pigments. From the results of molecular identification by 16S rDNA method, it was shown that bacterium TH8 was closely related to Bacillus licheniformis with 98% homology value.
关键词：Thalassia hemprichii ;carotenoids ;HPLC;Diadinoxanthin ;16S rDNA PCR